VitroVivo Special Staining Services
Special stains traditionally refer to any staining other than an H&E. It covers a wide variety of methods that may be used to visualize particular tissue structures, elements, or even micro-organisms not identified by H&E staining.
Other methods of staining such as immunohistochemistry or in situ hybridization used to target specific proteins or DNA/RNA sequences may be included as members of the “special stains”. However, since they are quite different in method and purpose, they could be separated into a third category know as “advanced stains”.
There can be hundreds of special stains for different purposes, but only a few are regularly used for research or clinical needs. Unlike H&E staining, special staining, due to their complexity, may have to be carried out by hands.
In contrast to “routine” stain services, special stains are used to differentiate specific components seen in the H&E-stained section. It is the application of various dyes to tissue sections prepared on a microscope slide. Special staining can be applied to highlight specific tissue components, microbes, metals, salts, and other artifacts.
VitroVivo offers the following special staining services with standard protocols:
- Mammary gland whole mount staining
- Alcian blue hematoxylin/orange G staining
- Oil Red O staining
- Modified Gomori’s trichrome staining
- Bielschowsky’s silver staining
- Masson’s trichrome staining
- Picro-sirius red staining
- Reticulum staining, and
- More custom services as per specific request. Please send your email for inquiry to: firstname.lastname@example.org
- VitroVivo has special stain kits available for the staining by yourself.
Frequently Asked Questions (FAQ)
FAQ 1. What is a special stain?
Any stain, other than an H&E stain, is classified as a special stain. The common special stains include mammary gland whole mount stain, alcian blue hematoxylin-orange G stain, alcian blue stain, alcian blue – PAS stain, oil red O stain, Nissl stain, Bielschowsky’s silver stain and Masson’s trichrome stain, etc. For more information, please visit our website page of Histochemical Stain Kits and Image Gallery.
FAQ 2. How do I choose VitroVivo special staining services?
This table gives you a guidance of the choice of special staining methods or kits (if you want to perform staining by your self). If you can not find the staining methods from this table, please send your email to email@example.com for inquiry, our service team will get back to you as soon as possible.
|Product Name||SKU#||Visualization for||Typical Results|
|Hematoxylin and Eosin Kit||VB-3000||General morphology of tissue and cell|
|Mammary Gland Whole Mount Stain Kit||VB-3001||Wholemount staining of mouse mammary glands|
|Alcian Blue Hematoxylin-Orange G Stain Kit||VB-3002||Differentiate cartilage, mature bone, and immature bone found in various stages of endochondral ossification and fracture callus|
|Alcian Blue Stain Kit||VB-3003||Tisssue mucosubstances|
|PAS Stain Kit||VB-3004||Glycogen, mucin, and fungi|
|Alcian Blue – PAS Stain Kit||VB-3005||Acidic and neutral mucins as well as mixtures of acidic and neutral mucins|
|Luxol Fast Blue Stain Kit||VB-3006||Myelin including phospholipids and neurons|
|Oil Red O Stain Kit||VB-3007||lipid and fat staining on formalin fixed frozen sections|
|Alizarin Red Stain Kit||VB-3008||Calcium on tissue sections|
|Prussian Blue Stain Kit||VB-3009||Ferric iron on tissue sections|
|Nissl Stain Kit||VB-3010||Neuron Nissl body|
|Congo Red Amyloid Stain Kit||VB-3011||Amyloid deposits|
|Sudan Black B Lipid Stain Kit||VB-3012||Lipid and fat|
|Toluidine Blue Stain Kit||VB-3013||Mast cells|
|Modified Gomori’s Trichrome Stain Kit||VB-3014||Connective fiber|
|Bielschowsky’s Silver Stain Kit||VB-3015||Axons, neurofibrillary tangles and senile plaques|
|Masson’s Trichrome Stain Kit||VB-3016||Collagen and mucus|
|Picro-Sirius Red Stain Kit||VB-3017||Collagen fibers|
|Reticulum Stain Kit||VB-3018||Reticular fibers|
|Verhoeff Van Gieson Elastin Stain Kit||VB-3019||Elastic fibers|
|Fontana-Masson Stain Kit||VB-3020||Melanin pigment and argentaffin granules|