VitroView™ Oil Red O Stain Kit

$195.00

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SKU: VB-3007 Category:

Description

Oil Red O is a lysochrome diazo dye used for detecting neutral triglycerides and lipids in tissue. It is performed on fresh frozen tissue sections or cultured cells. Oil Red O Stain Kit is used for lipid and fat staining on formalin fixed frozen sections. It is not suitable for paraffin embedded tissue sections.

Kit Contents

  • VB-3007-1 Pre-Stain Solution ———————– 100 ml
  • VB-3007-2 Oil Red O Solution ————————100 ml
  • VB-3007-3 Differentiation Solution————— 100 ml
  • VB-3007-4 Mayer’s Hematoxylin Solution —- 100 ml

Storage

Room temperature.

Protocol

  1. Cut frozen sections to 8 to10μm. Air dry the sections to the slides
  2. Fix in 10% buffered formalin for 20 mins at room temperature, briefly wash with running tap water for 3 mins.
  3. Place in Pre-Stain Solution for 2 mins
  4. Stain in pre-warmed Oil Red O solution for 8-10 minutes in 60 ºC oven
  5. Differentiate in pre-warmed Differentiation Solution for 2-5 minutes in 60 ºC oven
  6. Rinse in 2 water changes of distilled at room temperature.
  7. Lightly stain nuclei with Mayer’s Hematoxylin Solution for 5 dips
  8. Rinse with tap water for 3 mins
  9. Mount with aqueous mounting medium.

 Technical Points

  1. Include a positive control
  2. Cryostat sections 8 to 10 microns for formalin fixed smears. Formalin fixation gives good results.
  3. Step 9 – Obviously, the section must not be taken through clearing solvents prior to mounting, as this will remove the lipid to be detected.

Results

  • Lipid…………………………….. red
  • Nuclei…………………………….blue

Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.

User Manual and Material Safety Data Sheet (MSDS)  (PDF)

VB-3007 User manual

VB-3007 MSDS

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