VitroVivo has available 5′-aminoallyl dUTP and UTP analogs, as well as conjugates with biotin and various fluorescent dyes, which indicates our very bright and photostable Andy Fluor dyes.
Fluorescent and biotin-labeled dUTP and UTP are widely applied for enzymatic incorporation into DNA or RNA.
Biotin-11-dUTP can be enzymatically incorporated into DNA via nick translation, random priming. The number ’11’ is the number of atoms in the linker between biotin and dUTP. The resulting amine-containing DNA can be subsequently labeled with a fluorescent dye, biotin or other haptens via conventional peptide coupling methods.
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