VitroView™ Congo Red Amyloid Stain Kit

$195.00

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SKU: VB-3011 Category:

Description

Congo Red Amyloid Stain Kit is used for the detection of amyloid in formalin-fixed, paraffin-embedded tissue sections with amyloidosis, and may be used for frozen sections as well. . Amyloid deposits will stain red, while nuclei will stain blue. The thickness of sections is usually 5 µm. However, in cases containing low amyloid deposits, 10µm thick sections will give better results.

Kit Contents

  •    VB-3011-1 Congo Red Solution ——————–250 ml
  •    VB-3011-2 Alkaline Alcohol Solution ————250 ml
  •    VB-3011-3 Mayer’s Hematoxylin Solution — 250 ml

Storage Condition:

Store at room temperature. 

Protocol

  1. For paraffin-embedded tissue sections, deparaffinize and hydrate to 70% alcohol. Rinse in distilled water. For frozen sections, dry sections for 5 minutes. Fix sections in neutral formalin solution for 20 minutes. Rinse in distilled water.
  2. Stain in congo red solution for 15-20 minutes. 
  3. Rinse in distilled water. 
  4. Differentiate quickly (5-10 dips) in alkaline alcohol solution. 
  5. Rinse in tap water for 1 minute. 
  6. Counterstain with Mayer’s hematoxylin for 30 seconds. 
  7. Rinse in tap water for 2 minutes. 
  8. Dehydrate through 95% alcohol, 2 changes of 100% alcohol, 3 minutes each. 
  9. Clear with 3 changes of xylene (5 minute per change)
  10. Mount coverslip onto glass slide with Permount or some other suitable organic mounting medium.  

Results  

  • Amyloid, elastic fibers, eosinophil granules ——– red 
  • Nuclei ——————————————————– blue   

 Positive Controls 

Know amyloidosis containing tissue (i.e. Alzheimer’s disease). 

Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.

User Manual and Material Safety Data Sheet (MSDS)  (PDF)

VB-3011 User manual

VB-3011 MSDS

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