Description
The Verhoeff Elastic Masson Trichrome Stain offers a comprehensive method for simultaneously staining elastic, muscle, and connective tissue, making it ideal for routine use in histopathology. Verhoeff’s technique renders the elastica black, creating a distinct contrast with the red-stained muscle and green or blue-stained connective tissue, achieved through a modification of Masson’s trichrome. Additionally, cell nuclei exhibit reddish brown to blue-black hue with Weigert’s iron hematoxylin. This kit is particularly well-suited for examining vascular pathology in experimental pathology, surgical and autopsy sections.
Kit Components
| Sku# | Reagent Name | Volume (ml) |
| VB-3034-1 | Ethanol saturated with picric acid | 250 ml |
| VB-3034-2 | Verhoeff’s elastic stain solution A | 150 ml |
| VB-3034-3 | Verhoeff’s elastic stain solution B | 60 ml |
| VB-3034-4 | Verhoeff’s elastic stain solution C | 60 ml |
| VB-3034-5 | Biebrich scarlet-acid fuchsin solution | 250 ml |
| VB-3034-6 | Phosphotungstic/phosphomolybdic Acid Solution | 250 ml |
| VB-3034-7 | Aniline Blue Solution | 250 ml |
| VB-3034-8 | 1 % acetic acid solution | 250 ml |
Storage
Store at room temperature.
Protocol
- Deparaffinize in xylene (6 minutes) ×2
- Rehydrate: Ethanol 100% (2 minutes) ×2; Ethanol 95% (2 minutes) ×2; Ethanol 70% (2 minutes) ×1.
- Rinse in distilled water (2 minutes) ×1.
- Mordant in ethanol saturated with picric acid for 5 minutes
- Wash sections in tap water for 5 min until yellow color disappears.
- Freshly prepare modified Verhoeff’s elastic stain solution: mix Verhoeff’s elastic stain solution A, B and C at a ratio of 2.5:1:1.
- Stain in modified Verhoeff’s elastic stain for 6 min.
- Wash in running tap water for 10 min. Rinse in distilled water for 1 min.
- Stain in Biebrich scarlet-acid fuchsin solution for 2-3 min
- Place in Phosphotungstic/phosphomolybdic Acid Solution for 15 min. discard solution.
- Transfer directly into Aniline Blue Solution for 5 minutes.
- 1% Acetic acid for 2-3 seconds discard solution.
- Dehydrate with 2 changes of 95% Ethanol and 2 changes of 100% Ethanol (2 minute per change).
- Clear with 2 changes of xylene (5 minute per change).
- Mount cover slip onto glass slide with Permount or some other suitable organic mounting medium.
Control Tissue
Artery or lung tissue
Expected Results:
- Elastic fibers——————————blue-black
- Nuclei————————————–reddish brown to blue-black
- Muscle, keratin & cytoplasm ———-red
- Collagen———————————-green
References:
- Garvey W, et al (1984).Modified elastic tissue-masson trichrome stain. Stain Technology. 59 (3): 213-216
- Wu J, et al (2015). Full-Thickness Thermal Injury Delays Wound Closure in a Murine Model. Advances in Wound Care, 4(2):83-91
Note:
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Precautions:
Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
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