Description
The VitroView™ Von Kossa Calcium Stain Kit is a reliable and easy-to-use solution for detecting calcium deposits in tissue samples. Designed for use in paraffin-embedded sections, undecalcified bone, and calcium-rich tissues, this kit provides rapid and reproducible staining, highlighting calcium salts in black or brown-black, with nuclei counterstained in red and cytoplasm in pink.
Ideal for histology, pathology, and biomedical research, the VitroView™ Von Kossa kit enables high-contrast visualization of calcium deposition, supporting studies of bone development, mineralization disorders, and tissue calcification. With optimized reagents and clear instructions, this kit delivers consistent, high-quality results for both routine and advanced research applications.
Key Benefits
- Sensitive and specific detection of calcium salts
- Compatible with paraffin sections and undecalcified tissues
- Quick and reproducible staining
- Optimized for both Sunlight and standard laboratory lighting
Kit Contents
| VB-3043-1 | Silver Nitrate Solution | 100 ml |
| VB-3043-2 | Sodium Thiosulfate Solution | 100 ml |
| VB-3043-3 | Nuclear Fast Red Solution | 100 ml |
Storage Condition:
Silver Nitrate Solution : Store at 2–8°C.
The others: Store at room temperature.
Staining Procedures for FFPE Tissue Sections
-
- Deparaffinize in xylene I for 6 minutes and II for 6 minutes.
- Rehydrate
- Ethanol 100% (2 minutes)
- Ethanol 100% (2 minutes)
- Ethanol 95% (2 minutes)
- Ethanol 95% (2 minutes)
- Rinse sections in distilled water for 5 minutes.
- Cover the sections with 0.5-1 ml of Silver Nitrate Solution and expose them to bright sunlight through a clear glass window for 20 minutes. Alternatively, place the sections under a 60–100 watt light bulb for 1 hour, or until the calcium turns black.
Note: If the stain is weak or fades, this indicates insufficient light exposure. In such cases, extend the incubation time as needed, up to several hours.
- Rinse the sections thoroughly with multiple changes of distilled water.
- To remove any unreacted silver, cover the sections with 0.5-1 ml of sodium thiosulfate and let it sit for 5 minutes.Rinse thoroughly in distilled water.
- Counterstain with nuclear fast red for 5 minutes.
- Rinse in distilled water.
- Dehydrate with 2 changes of 95% Ethanol and 2 changes of 100% Ethanol (2 minute per change).
- Clear with 3 changes of xylene (5 minute per change)
- Mount coverslip onto glass slide with Permount or some other suitable organic mounting medium
Expected Results
- Calcium salts———Black or brown-black
- Nuclei——————Red
- Cytoplasm————Pink
More Images
Precautions
- Handle reagents with care.
- Avoid contact with eyes, skin, or clothing.
- Do not ingest.
- Always wear gloves when handling chemicals.
