IHC Hematoxylin Counterstain kit is designed to be used after completion of immunohistochemical staining. The counterstaining color will be light blue in nuclei.
RTU Hematoxylin Solution 15ml ×2 (for 300 slides)
Store at room temperature.
Recommended Assay Procedure for Immunohistochemistry
- After DAB staining, incubate sections with 3 drops of RTU hematoxylin solution for 1-2 minutes
- Rinse in tape water 2×2 min.
- Dehydrate through 75% ethanol for 2 min, 95% ethanol for 2 min, and 100% ethanol for 2x3min. Clear in xylene for 2×5min.
- Coverslip with mounting medium.
Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
Handle with care in chemical hood. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
User Manual (PDF)