Description
A combined Verhoeff’s elastic tissue Masson technique is presented which stains elastic fibers of all sizes, nuclei and connective tissue in a single tissue section. This kit is useful in identifying normal tissue morphology as well as heart, liver, lung and kidney pathologic conditions.
Kit Components
| Sku# | Reagent Name | Volume (ml) |
| VB-3034-1 | Ethanol saturated with picric acid | 250 ml |
| VB-3034-2 | Verhoeff’s elastic stain solution A | 150 ml |
| VB-3034-3 | Verhoeff’s elastic stain solution B | 100 ml |
| VB-3034-4 | Verhoeff’s elastic stain solution C | 50 ml |
| VB-3034-5 | Biebrich scarlet-acid fuchsin solution | 250 ml |
| VB-3034-6 | Phosphotungstic acid solution | 250 ml |
| VB-3034-7 | Light green Solution | 250 ml |
| VB-3034-8 | 1 % acetic acid solution | 250 ml |
Storage
Store at room temperature.
Protocol
- Deparaffinize in xylene (6 minutes) ×2
- Rehydrate:
- Ethanol 100% (2 minutes)×2
- Ethanol 95% (2 minutes) ×2
- Ethanol 70% (2 minutes) ×1
- Rinse in distilled water (2 minutes) ×1
- Mordant in ethanol saturated with picric acid for 5 minutes
- Wash sections in tap water for 5 min until yellow color disappears.
- Freshly prepare modified Verhoeff’s elastic stain solution: mix Verhoeff’s elastic stain solution A, B and C at a ratio of 3:2:1.
- Stain in modified Verhoeff’s elastic stain for 6 min.
- Wash in running tap water for 10 min.
- Stain in Biebrich scarlet-acid fuchsin solution for 2-3 min.
- Rinse in distilled water.
- Place in phosphotungstic acid solution for 15 min.
- Count stain in light green solution for 3 min.
- Differentiate in 1 % acetic acid for 3 min.
- Dehydrate with 2 changes of 95% Ethanol and 2 changes of 100% Ethanol (2 minute per change).
- Clear with 2 changes of xylene (5 minute per change).
- Mount cover slip onto glass slide with Permount or some other suitable organic mounting medium.
Control Tissue: Artery
Expected Results:
- Elastic fibers——————————blue-black
- Nuclei————————————–reddish brown to blue-black
- Muscle, keratin & cytoplasm ———-red
- Collagen———————————-green
References:
- Garvey W, et al (1984).Modified elastic tissue-masson trichrome stain.Stain Technology. 59 (3): 213-216
- Wu J, et al (2015). Full-Thickness Thermal Injury Delays Wound Closure in a Murine Model. Advances in Wound Care, 4(2):83-91
Note:
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Precautions:
Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
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User Manual and Material Safety Data Sheet (MSDS) (PDF)
VB-3034 MSDS
