VitroView™ Verhoeff Elastic Masson Trichrome Stain Kit


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SKU: VB-3034 Category:


The Verhoeff Elastic Masson Trichrome Stain offers a comprehensive method for simultaneously staining elastic, muscle, and connective tissue, making it ideal for routine use in histopathology. Verhoeff’s technique renders the elastica black, creating a distinct contrast with the red-stained muscle and green or blue-stained connective tissue, achieved through a modification of Masson’s trichrome. Additionally, cell nuclei exhibit reddish brown to blue-black hue with Weigert’s iron hematoxylin. This kit is particularly well-suited for examining vascular pathology in experimental pathology, surgical and autopsy sections.

Kit Components

Sku# Reagent Name Volume (ml)
VB-3034-1 Ethanol saturated with picric acid 250 ml
VB-3034-2 Verhoeff’s elastic stain solution A 150 ml
VB-3034-3 Verhoeff’s elastic stain solution B 60 ml
VB-3034-4 Verhoeff’s elastic stain solution C 60 ml
VB-3034-5 Biebrich scarlet-acid fuchsin solution 250 ml
VB-3034-6 Phosphotungstic/phosphomolybdic Acid Solution 250 ml
VB-3034-7 Aniline Blue Solution 250 ml
VB-3034-8 1 % acetic acid solution 250 ml


Store at room temperature.


  1. Deparaffinize in xylene (6 minutes) ×2
  2. Rehydrate: Ethanol 100% (2 minutes) ×2; Ethanol 95% (2 minutes) ×2; Ethanol 70% (2 minutes) ×1.
  3. Rinse in distilled water (2 minutes) ×1.
  4. Mordant in ethanol saturated with picric acid for 5 minutes
  5. Wash sections in tap water for 5 min until yellow color disappears.
  6. Freshly prepare modified Verhoeff’s elastic stain solution: mix Verhoeff’s elastic stain solution A, B and C at a ratio of 2.5:1:1.
  7. Stain in modified Verhoeff’s elastic stain for 6 min.
  8. Wash in running tap water for 10 min. Rinse in distilled water for 1 min.
  9. Stain in Biebrich scarlet-acid fuchsin solution for 2-3 min
  10. Place in Phosphotungstic/phosphomolybdic Acid Solution for 15 min. discard solution.
  11. Transfer directly into Aniline Blue Solution for 5 minutes.
  12. 1% Acetic acid for 2-3 seconds discard solution.
  13. Dehydrate with 2 changes of 95% Ethanol and 2 changes of 100% Ethanol (2 minute per change).
  14. Clear with 2 changes of xylene (5 minute per change).
  15. Mount cover slip onto glass slide with Permount or some other suitable organic mounting medium.

Control Tissue

Artery or lung tissue

Expected Results:

  • Elastic fibers——————————blue-black
  • Nuclei————————————–reddish brown to blue-black
  • Muscle, keratin & cytoplasm ———-red
  • Collagen———————————-green


  1. Garvey W, et al (1984).Modified elastic tissue-masson trichrome stain. Stain Technology. 59 (3): 213-216
  2. Wu J, et al (2015). Full-Thickness Thermal Injury Delays Wound Closure in a Murine Model. Advances in Wound Care, 4(2):83-91


This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.


Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.

More Images

Mouse Lung, Elastic Masson Trichrome Stain, 20x
Mouse Bronchus, Elastic Masson Trichrome Stain, 20x
Mouse Kidney, Elastic Masson Trichrome Stain, 40x

User Manual and Material Safety Data Sheet (MSDS)  (PDF)

VB-3034-User Manual

VB-3034 MSDS