A combined Verhoeff’s elastic tissue Masson technique is presented which stains elastic fibers of all sizes, nuclei and connective tissue in a single tissue section. This kit is useful in identifying normal tissue morphology as well as heart, liver, lung and kidney pathologic conditions.
|Sku#||Reagent Name||Volume (ml)|
|VB-3034-1||Ethanol saturated with picric acid||250 ml|
|VB-3034-2||Verhoeff’s elastic stain solution A||150 ml|
|VB-3034-3||Verhoeff’s elastic stain solution B||100 ml|
|VB-3034-4||Verhoeff’s elastic stain solution C||50 ml|
|VB-3034-5||Biebrich scarlet-acid fuchsin solution||250 ml|
|VB-3034-6||Phosphotungstic acid solution||250 ml|
|VB-3034-7||Light green Solution||250 ml|
|VB-3034-8||1 % acetic acid solution||250 ml|
Store at room temperature.
- Deparaffinize in xylene (6 minutes) ×2
- Ethanol 100% (2 minutes)×2
- Ethanol 95% (2 minutes) ×2
- Ethanol 70% (2 minutes) ×1
- Rinse in distilled water (2 minutes) ×1
- Mordant in ethanol saturated with picric acid for 5 minutes
- Wash sections in tap water for 5 min until yellow color disappears.
- Freshly prepare modified Verhoeff’s elastic stain solution: mix Verhoeff’s elastic stain solution A, B and C at a ratio of 3:2:1.
- Stain in modified Verhoeff’s elastic stain for 6 min.
- Wash in running tap water for 10 min.
- Stain in Biebrich scarlet-acid fuchsin solution for 2-3 min.
- Rinse in distilled water.
- Place in phosphotungstic acid solution for 15 min.
- Count stain in light green solution for 3 min.
- Differentiate in 1 % acetic acid for 3 min.
- Dehydrate with 2 changes of 95% Ethanol and 2 changes of 100% Ethanol (2 minute per change).
- Clear with 2 changes of xylene (5 minute per change).
- Mount cover slip onto glass slide with Permount or some other suitable organic mounting medium.
Control Tissue: Artery
- Elastic fibers——————————blue-black
- Nuclei————————————–reddish brown to blue-black
- Muscle, keratin & cytoplasm ———-red
- Garvey W, et al (1984).Modified elastic tissue-masson trichrome stain.Stain Technology. 59 (3): 213-216
- Wu J, et al (2015). Full-Thickness Thermal Injury Delays Wound Closure in a Murine Model. Advances in Wound Care, 4(2):83-91
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
User Manual and Material Safety Data Sheet (MSDS) (PDF)