Description
Fluorescence microscopy is a cornerstone of modern biological research, enabling detailed visualization of cellular and subcellular structures. However, maintaining fluorescence signal stability over time remains a critical challenge due to photobleaching and sample degradation. The VitroView™ Permanent Aqueous Mounting Medium with DAPI is an advanced, ready-to-use mounting solution designed to preserve fluorescence integrity while providing optimal specimen clarity and long-term stability.
This aqueous-based mounting medium is formulated with strong anti-fade agents to protect fluorescent labels from rapid photobleaching, ensuring consistent and durable signal retention for repeated imaging and archival purposes. Additionally, it contains DAPI (4′,6-diamidino-2-phenylindole), a widely used nuclear counterstain that binds to DNA, allowing for easy identification of cell nuclei in blue fluorescence.
Compatible with a broad range of fluorescent probes and antibodies, VitroView™ Permanent Aqueous Mounting Medium offers excellent refractive index matching, minimal background autofluorescence, and superior specimen protection. It is ideal for applications such as immunofluorescence (IF), immunohistochemistry (IHC), and fluorescence in situ hybridization (FISH), providing researchers with reliable, high-contrast imaging results.
With its permanent sealing properties, this mounting medium ensures long-term sample preservation, making it an indispensable tool for laboratories requiring consistent, high-quality fluorescence microscopy data.
Application
Compatible with AEC, DAB, Fast Red, Oil Red, BCIP/NBT, BCIP/INT and fluorescent dyes and phycobiliproteins.
Package Size
15ml/ squeeze bottle
Storage
Product is stable for about 12 months at 2-8 ºC.
Usage Instructions
- Place slides flat on paper towel on countertop with specimen facing up.
- Blot excess water from the slide without letting tissue specimen dry prior to mounting.
- Apply 1-2 drops of Anti-Fade Permanent Aqueous Mounting Medium to completely cover the specimen with a thin layer.
- Place a cover slip on the liquid and let sit at room temperature for 45 minutes to 1 hour to allow drying..
Precautions
- Avoid exposure of VitroView™ Anti-fade Permanent Aqueous Mounting Medium to moisture or light, as this may affect its performance.
- Use appropriate personal protective equipment (PPE) when handling chemicals and biological specimens.
- Dispose of unused VitroView™ VitroView™ Anti-fade Permanent Aqueous Mounting Medium according to local regulations and guidelines.
Disclaimer:
This user manual serves as a general guideline. Users should adapt procedures based on specific experimental requirements and equipment specifications.
