Genomic DNA Extracted from Pure Human Liver Normal Cells Isolated by LCM from FFPE Sample

Description

Sample Basic Information

Tissue ID	Organ	Pathology Diagnosis	Gender	Age	Grade	TMN	IHC Data	Sample Format
Hu-08002A	Human  Liver	Normal liver tissue	Male	42	N/A	N/A	Ki67 IHC	FFPE

Tissue H&E Staining and IHC images

H&E Stain

Ki67 IHC

Flowchart of LCM  for The Product Preparation

1. FFPE samples are cut at 6 microns and mounted onto pen membrane glass slides and let the slides dry for 2 hours at room temperature.
2.  After deparaffinization and rehydration, quick H&E staining is performed.
3. Arcturus XT Laser Microdissection System is used for LCM. Microdissection is performed using IR and UV laser and CapSure Macro LCM Cap.
4. The total dissected cells may be varied between 10,000 to 50,000 cells which depend on tissue type. The DNA is extracted from Caps by using Arcturus® PicoPure® DNA Extraction Kit (Thermo Fisher, KIT0103).
5. Extracted DNA is quantized by UV adsorption in a NanoDrop.

Quality Control

• The tissue H&E staining slides were examined by certified pathologists. Pathological re-confirmation report is generated and digital image captured. 
• LCM is supervised by our certified pathologist.
• Three example LCM images including Before, After LCM, and LCM cap images from LCM tissue slides are took to demonstrate the dissected cells.

Product Format and Size

          100 ng of DNA

Applications

• DNA  from pure cell population microdessected from FFPE tissues can be used for PCR, hybridization, sequencing and epegenectics, etc. 
• The product is only used for biomedical research. It is not used for clinical diagnosis.

Storage

        Store at 4-8ºC for shot time and  at -80ºC for longer time

References

1. Longuespée R, et al. A laser microdissection-based workflow for FFPE tissue microproteomics: Important considerations for small sample processing, Methods;2016, 104: 154-162
2. Cai J, et al. The Use of Laser Microdissection in the Identification of Suitable Reference Genes for Normalization of Quantitative Real-Time PCR in Human FFPE Epithelial Ovarian Tissue Samples. PLoS ONE 2014, 9(4): e95974. 
3. Drummond ES, et al. Proteomic analysis of neurons microdissected from formalin-fixed, paraffin-embedded Alzheimer’s disease brain tissue.Sci Rep. 2015;5:15456
4. Bockmeyer CL, et al. Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples. BMC Mol Biol. 2018;19(1):2
5. Murray, Graeme I. (Ed.). Laser Capture Microdissection (Methods and Protocols, © 2018

Product Data Sheet (PDF)

LCM-D08002A Data Sheet