Fluorescent Conjugated Secondary Antibodies: Enhance Detection Sensitivity in Biological Research
Fluorescent conjugated secondary antibodies are essential tools in modern biological research, especially in studies that involve target antigens and primary antibodies. These antibodies are crucial for detecting specific components within complex biological systems with high sensitivity. Commonly used in applications such as imaging, flow cytometry, and western blotting, fluorescent secondary antibodies offer unmatched performance in visualizing and analyzing proteins, cells, and other biological targets.
Secondary antibodies are key for indirect detection, as they bind to primary antibodies that, in turn, bind to the target antigen. This approach enhances sensitivity through signal amplification, as multiple secondary antibodies bind to a single primary antibody, providing a stronger and more detectable signal compared to direct detection with primary antibodies alone.
Fluorescent secondary antibodies are widely used for fluorescent cell imaging, western blotting, immunostaining, and various other applications that require precise and sensitive detection.
At VitroVivo, we offer a wide range of fluorescent dye-conjugated secondary antibodies designed to meet the diverse needs of researchers. Our products feature excellent brightness and photostability, ensuring optimal performance in your experiments.
Key Features of VitroVivo Fluorescent Conjugated Secondary Antibodies:
- Bright Fluorescence: Exceptional signal intensity with minimal background interference, comparable to VitroView™ fluorescent conjugates.
- Superior Photostability: Resistant to photobleaching for long-lasting performance.
- Variety of Fluorophores: Multiple FL dye options for multicolor imaging and complex experiments.
- Instrument Compatibility: Tailored excitation and emission spectra to match the laser settings and emission filters of popular fluorescence instruments.
Selecting the correct secondary antibody is simple: choose one that recognizes the host species of your primary antibody. However, choosing the optimal secondary antibody for your research requires an understanding of your specific detection assay.
Enhance your research with VitroVivo’s fluorescent conjugated secondary antibodies for precise, sensitive, and reliable antigen detection across various applications.