Biotin-11-dUTP can be enzymatically incorporated into DNA with Reverse Transcriptases, Taq DNA Polymerase, phi29 DNA Polymerase, Klenow Fragment, exo-, Klenow Fragment, and DNA Polymerase I. The number ’11’ is the number of atoms in the linker between biotin and dUTP.
50µl (1 mM solution in TE buffer).
Store at -20°C
Enzymatic non-radioactive labeling of DNA by PCR, nick-translation, cDNA synthesis, random primed labeling, or primer extension.