Description
Although neutral buffered formalin (NBF) is rendered as the most widely used fixative in clinical pathology and routine diagnostics, a major drawback of NBF fixation is its detrimental effect on DNA/RNA quality and its denature of proteins thereby hampering immunohistochemical visualization of certain antigens. The VitroView™ Zinc-based Formalin-Free Fixative Solution offers a better, convenient and safer alternative formalin-free strategy to neutral buffered formalin (NBF) for the preservation of tissue specimens.
Key Advantage
1. Safer to Handle
• Non-carcinogenic and low toxicity compared with formaldehyde
• Reduced fumes and odors → better lab air quality
• Fewer regulatory and disposal concerns
2. Excellent Antigen Preservation
• Zinc salts stabilize proteins without extensive cross-linking
• Often superior results for:Immunohistochemistry (IHC), mmunofluorescence, Less need for harsh antigen-retrieval steps
3. Better Molecular Integrity
• Improved preservation of: DNA, RNA and Proteins
• More compatible with downstream techniques like PCR and sequencing than formalin-fixed tissues
4. Good Morphological Detail
• Maintains clear cellular and nuclear structure
• Comparable (and sometimes superior) histology to neutral buffered formalin
5. Faster Fixation
• Zinc penetrates tissue quickly
• Useful when rapid processing is important
6. Environmentally Friendlier
• Easier waste management
• Reduced environmental and occupational hazards
Package Size
1000ml/ bottle
Storage
At room temperature.
Protocols
1. Tissue Preparation: Freshly harvested tissue should be cut into smaller pieces (maximum 4-5mm) to ensure adequate penetration of the fixative. This helps in achieving uniform fixation.
2. Fixation: Submerge the tissue completely in the Zinc-based Formalin-Free Fixative Solution . Use enough amount of fixation solution to ensure that the tissue is fully immersed.
3. Fixation Time: The fixation time should not exceed 24-48 hours, depending on the size and type of tissue. Prolonged fixation can lead to over-fixation, which may hinder subsequent processing and staining.
4. Post-Fixation: After fixation, transfer the tissue to PBS or 70% ethanol for further processing. This step is crucial for preparing the tissue for embedding and sectioning.
Important Notes
- This product is intended for research use only. Not for use in diagnostic or therapeutic procedures in humans or animals.
- Handle reagents with care. Avoid contact with eyes, skin, and clothing.
- Do not ingest.
- Always wear appropriate protective equipment, such as gloves and safety goggles.
- Perform the experiment in chemical hood.
Disclaimer
This user manual serves as a general guideline. Users should adapt procedures based on specific experimental requirements and equipment specifications.
User Manual and Material Safety Data Sheet (MSDS) (PDF)
VB-9006 MSDS
