Description
The DNase Set for RNA Isolation is designed to remove contaminating genomic DNA during RNA purification procedures. By applying DNase I directly to the purification column, residual DNA is efficiently digested without affecting RNA integrity or yield. This on-column DNase treatment helps ensure that the isolated RNA is free of genomic DNA contamination, which is critical for downstream applications such as RT-PCR, qPCR, RNA sequencing, and gene expression analysis. The DNase Set is optimized for convenient integration into standard column-based RNA isolation workflows.
Kit Contents
| Buffer VDD | 5 ml |
| DNase I Powder | 1 mg |
Storage
Store DNase I powder at -20°C. After reconsidering DNase I, aliquot the solution and store it at -20°C.
Typical Procedures
Preparation of DNase I Stock Solution
Add 550 µl of RNase-Free Water to 1 mg of DNase I powder and gently mix by inverting the vial. After reconstitution, aliquot the DNase I solution and store the aliquots at −20 °C. Avoid repeated freeze–thaw cycles to maintain enzyme stability.
Removal of DNA During RNA Isolation
To remove DNA during RNA isolation, gently mix 10 µl of DNase I stock solution with 70 µl of Buffer VDD and apply the mixture directly onto the column membrane. Incubate at 20–30 °C for 15 minutes. Please follow the protocol when using this set with Kits VB-5002, VB-5003, VB-5005, and VB-5006.
User Manual and Material Safety Data Sheet (MSDS) (PDF )
VB-5007 MSDS
Note
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Precautions
Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
