Description
The Twort’s Counterstaining Kit is designed for histological procedures to provide contrast for easier visualization of tissue sections. It can be used as a counterstain for Gram staining.
Kit Contents
| VB-2001-1 | Twort’s Neutral Red Solution | 200 ml |
| VB-2001-2 | Twort’s Fast Green Solution | 100 ml |
Storage
Room temperature.
Procedure
Things to do before staining:
Preparation of Working Twort’s counterstain solution:
- Twort’s Neutral Red Solution—–10 ml
- Twort’s Fast Green Solution——5 ml
- ddH2o————————————-25 ml
Mix immediately before use.
Protocol
- Deparaffinize sections if necessary and hydrate to distilled water.
- Stain slide in Working Twort’s Counterstain for 5 min. Use once and discard.
- Rinse quickly in distilled water and carefully blot dry.
- Dehydrate slides quickly in clean pure Acetone with 3 dips (do not use any alcohols).
- Clear in three changes of xylene, 10 dips each; coverslip with compatible mounting medium
Expected Results
- Nuclei——————————–Red
- Cytoplasm—————————Light Green
More Images
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Note
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Precautions
Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
