DNA from Pure Cell Population

To extract DNA from pure cell population, FFPE samples are cut at 6 microns and mounted onto pen membrane glass slides and let the slides dry for 2 hours at room temperature. After deparaffinization and rehydration, quick H&E staining is performed. Arcturus XT Laser Microdissection System Tissue is used for LCM. Microdissection is performed using IR and UV laser and CapSure Macro LCM Cap. The dissected cells are on the film of Macro LCM Cap. The total dissected cells may be varied between 10,000 to 50,000 cells from different tissue type. DNA is extracted from Macro LCM Cap.