In Situ Detection of Autophagy

Autophagy, also known as autophagocytosis, is a catabolic mechanism that involves the degradation of unnecessary or dysfunctional cellular components through the actions of lysosomes. The evaluation of autophagy in situ requires indispensable tools such as transmission electron microscopy (TEM) and immunohistochemistry. Among the most reliable markers of the autophagic process, LC3B is currently considered to be one of the best.