Description
VitroView™ Biotin Donkey Anti-Goat IgG (H+L) Antibody is prepared by labeling high quality Donkey anti-goat IgG (H+L) with Biotin. Biotin label is used to subsequent detection by streptavidin for signal amplification.
Features
| Form Information | Specifications | |||
| Physical State | Lyophilized powder | Host/Isotype | Donkey | |
| Shipping Condition | Ambient | Class & Form | Polyclonal/Whole Antibody | |
| Purification | Immunoaffinity chromatography | Type | Secondary Antibody | |
| Buffer | PBS, pH 7.4, with 0.1% BSA | Tested Species Reactivity | goat | |
| Preservative | 0.02% sodium azide | Target Class | IgG (H+L) | |
| Storage Conditions | 2-8ºC | Label | Biotin | |
| Package size | 200 µg /200µl buffer | Excitation/Emission | —— | |
Reconstitution and Storage
Product is stable for about 12 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
Applications
- Western Blot (WB) 1:5,000 – 1:20,000
- Immunocytochemistry (ICC) 1:100 – 1:2,000
- Immunohistochemistry (IHC) 1:100 – 1:2,000
- Flow Cytometry (FACS) 1:100 – 1:1,000
