Description
Weigert’s Hematoxylin is an iron-mordanted hematoxylin stain. Ferric ions function as both:
- Oxidizing agent
- Mordant
The resulting iron-hematoxylin complex binds strongly to nuclear chromatin and is resistant to decolorization by acidic dyes, making it superior to alum hematoxylins in many special staining protocols.
The VitroView™ Weigert’s Hematoxylin Stain Kit is designed for staining cell nuclei in paraffin-embedded and frozen tissue sections. The stain is highly resistant to acidic solutions and is commonly used as the nuclear stain in: Masson’s Trichrome, Martius Scarlet Blue (MSB) and various connective tissue staining procedures.
The stain produces crisp blue-black to black nuclei while maintaining excellent resistance during subsequent acidic staining steps.
Application
- Masson’s Trichrome
- Martius Scarlet Blue (MSB)
- Various connective tissue staining procedures
Kit Contents
| SKU | Name | Volume |
| VB-3050W-1 | VitroView™ Weigert’s Hematoxylin Solution A | 500 ml |
| VB-3050W-2 | VitroView™ Weigert’s Hematoxylin Solution B | 500 ml |
Storage Condition
Room temperature.
Protocol
Weigert’s Hematoxylin Staining for Paraffin Sections
- Deparaffinize in xylene I for 6 minutes and II for 6 minutes.
- Rehydrate: ethanol 100% (2 minutes)x2; ethanol 95% (2 minutes)x2; ethanol 70% (2 minutes)x1; Rinse in distilled water (5 minutes).
- Follow the protocol of Masson’s Trichrome, Martius Scarlet Blue (MSB) or Various connective tissue staining procedures
Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
