Description
VitroView™ Eosin Solution is a premium, specially formulated counterstain designed to deliver vivid, high-contrast cytoplasmic visualization in routine histology and cytology applications. Primarily used as the counterstain in the foundational Hematoxylin and Eosin (H&E) staining technique, it provides a clear diagnostic contrast.
Key Features
- Vivid Cellular Contrast: Seamlessly highlights cell cytoplasm, collagen, and erythrocytes in striking, distinct shades of red and pink.
- Streamlined Workflow: Formulated to provide crisp staining with exceptionally low background interference.
- Ready-to-Use Convenience: Optimized to function smoothly without complex preparation steps, accelerating laboratory turnaround time.
- Ambient Stability: Designed for hassle-free storage at room temperature, eliminating the need for cold-chain refrigeration.
Ideal For
- Routine H&E Staining: Provides the essential secondary counterstain to Hematoxylin for broad tissue morphology evaluations.
- Research & Histopathology: Ideal for deep cell structure analysis across diverse paraffin-embedded or frozen biological tissue sections.
Kit Contents
VB-3000L-2 VitroView™ Eosin Solution – 500 ml
Storage Condition
Room temperature.
Protocol
H&E Stain for Paraffin Sections
- Deparaffinize in xylene I for 6 minutes and II for 6 minutes.
- Rehydrate: ethanol 100% (2 minutes)x2; ethanol 95% (2 minutes)x2; ethanol 70% (2 minutes)x1; Rinse in distilled water (5 minutes).
- Place in Mayer’s Hematoxylin Solution for 5 minutes.
- Rinse in tap water for 5 min with 3 changes. Or Bluing reagent for 30–60 seconds.
- Place in 95% Ethanol for 1 minute (DO NOT RINSE).
- Place in Eosin for 1 min 30sec.
- Dehydrate with 2 changes of 95% Ethanol and 2 changes of 100% Ethanol (2 minutes per change).
- Clear with 3 changes of xylene (5 minutes per change)
- Mount coverslip onto glass slide with Permount or some other suitable organic mounting medium.
H&E Stain for Frozen Sections
- Frozen section are fixed in acetone at -20 °C for 3 min or 80% Methanol at 4°C for 5 min
- PBS x 10 min.
- Rinse in DI water (2 minutes).
- Follow step 3-9 above.
Note: This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
