Sample Basic Information
|Tissue ID||Organ||Pathology Diagnosis||Gender||Age||Grade||TMN||IHC Data||Sample Format|
|Hu-02001T||Human breast||Breast invasive ductal carcinoma||Female||45||2||N/A||ER(80% +), PR(70%+), E-cad(+), P120(+), CK5/6(-), CK8/18(+), Ki67(15% +), Her2 (-)||FFPE|
Tissue H&E Staining and IHC images
Ki 67 IHC
Flowchart of LCM for The Product Preparation
- FFPE samples are cut at 6 microns and mounted onto pen membrane glass slides and let the slides dry for 2 hours at room temperature.
- After deparaffinization and rehydration, quick H&E staining is performed.
- Arcturus XT Laser Microdissection System is used for LCM. Microdissection is performed using IR and UV laser and CapSure Macro LCM Cap.
- The microdissected cells are on the film of Macro LCM Cap. The total dissected cells may be varied between 10,000 to 50,000 cells which depend on tissue type.
- Customers can be isolated DNA, RNA or protein from LCM Caps for the downstream applications.
- The tissue H&E staining slides are examined by certified pathologists. Pathological re-confirmation report is generated and digital image captured.
- LCM is supervised by our certified pathologist.
- Three example LCM images including Before, After LCM, and LCM cap images from LCM tissue slides are taken to demonstrate the dissected cells
Product Format and Size
- Dissected Cells on the film of Macro LCM Caps
- 2 LCM macro caps
Extraction Protocol for DNA, RNA and Protein from LCM Caps
- For DNA extraction, you can use Arcturus® PicoPure® DNA Extraction Kit (Thermo Fisher, KIT0103)
- For RNA isolation, you can use Arcturus® Paradise® PLUS FFPE RNA Isolation Kit (Thermo Fisher, Cat# KIT03121)
- For protein extraction, you can follow the protocol from Longuespée R, et al. A laser microdissection-based workflow for FFPE tissue microproteomics: Important considerations for small sample processing, Methods;2016, 104: 154-162
- The pure cell population on the film of caps can be used for extraction of DNA, RNA/miRNA or protein for RT-PCR, cDNA array, sequencing, epegenectics, proteomics and antibody array, etc.
- The product is only used for biomedical research. It is not used for clinical diagnosis.
Store at -80ºC
- Longuespée R, et al. A laser microdissection-based workflow for FFPE tissue microproteomics: Important considerations for small sample processing, Methods;2016, 104: 154-162
- Cai J, et al. The Use of Laser Microdissection in the Identification of Suitable Reference Genes for Normalization of Quantitative Real-Time PCR in Human FFPE Epithelial Ovarian Tissue Samples. PLoS ONE 2014, 9(4): e95974.
- Drummond ES, et al. Proteomic analysis of neurons microdissected from formalin-fixed, paraffin-embedded Alzheimer’s disease brain tissue.Sci Rep. 2015;5:15456
- Bockmeyer CL, et al. Recommendations for mRNA analysis of micro-dissected glomerular tufts from paraffin-embedded human kidney biopsy samples. BMC Mol Biol. 2018;19(1):2
- Murray, Graeme I. (Ed.). Laser Capture Microdissection (Methods and Protocols), © 2018