Description
Vitroview™ FL647 conjugated streptavidin is prepared by covalently labeling streptavidin with FL647 dye. Streptavidin has high binding affinity with biotin, so streptavidin conjugate is commonly used together with biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules. Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.
Specification
| Conjugate | FL647 |
| Physical State | 1.0 mg/ml (liquid) |
| Buffer | PBS, pH 7.4 |
| Preservative | 0.02% sodium azide |
| Stabilizer | 0.1% BSA |
| Excitation/ Emission | 647/664 nm |
Package Size
200 µg in 200µl buffer
Application
- Immunofluorescence microscopy
- In Situ Apoptosis (TUNEL) assay, visualized with fluorescence
- In Situ hybridization
- Flow cytometry
- Tetramer staining
- ELISA
- Microarrays
- Western blot
Reconstitution and Storage
Product is stable for about 6 months at 2- 8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
Guidelines for Use
Centrifuge the reconstituted protein conjugate solution briefly in a microcentrifuge before use. Add only the supernatant to the experiment. This step eliminates any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled streptavidin, a final concentration of 1–10 μg/mL should be satisfactory for most immunohistochemical applications. For flow cytometry applications, 0.06–1.0 μg per 1 × 106 cells should yield satisfactory result.
User Manual and Material Safety Data Sheet (MSDS) (PDF)
Note
This product is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.
Precautions
Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.
