VitroView™ Masson’s Trichrome Stain Kit

Description

Masson’s Trichrome Stain Kit is used to differentiate between collagen and smooth muscle in tumors, and the increase of collagen in diseases such as cirrhosis. It is a routine stain for liver and kidney biopsies.  This kit can be used for FFPE and frozen tissue slides.

Kit Introduction Viedeo

Kit Contents

• VB-3016-1 Bouin’s solution———————————————————250 ml
• VB-3016-2 Weigert’s Hematoxylin Solution A——————————125 ml
• VB-3016-3 Weigert’s Hematoxylin Solution B——————————125 ml
• VB-3016-4 Biebrich Scarlet-Acid Fuchsin Solution———————–250 ml
•  VB-3016-5 Phosphomolybdic-Phosphotungstic Acid Solution —250 ml
• VB-3016-6 Aniline Blue Solution————————————————-250 ml
• VB-3016-7 1% Acetic Acid Solution———————————————250 ml

Protocol

For formalin-fixed, paraffin-embedded (FFPE) tissue sections:

1. Deparaffinize in Xylene I for 6 minutes and II for 6 minutes.
2. Rehydrate: ethanol 100% (2 minutes)x2; ethanol 95% (2 minutes)x2; ethanol 70% (2 minutes)x1; Rinse in distilled water (5 minutes).Mordant in Bouin’s Solution, 60°C for 1 hour.
3. Prepare Weigert’s working hematoxylin: mix Weigert’s Hematoxylin Solution A and B at 1:1 ratio.
4. Weigert’s working hematoxylin for 10 minutes. (Note: Discard after use.)
5. Blue in running tap water for 5 minutes, rinse once for 1 min in distilled water.
6. Biebrich Scarlet-Acid Fuchsin Solution for 5 minutes. (Note: Solution may be used twice only, then discarded.)
7. Rinse once for 1 min in distilled water.
8. Phosphotungstic/phosphomolybdic acid solution for 10 minutes, discard solution.
9. Transfer directly into Aniline Blue Solution for 10 minutes.
10. Rinse once for 2-3 seconds in distilled water.
11. Acetic Acid Solution for 2-3 seconds, discards solution.
12. Dehydrate with 2 changes of 95% Ethanol and 2 changes of 100% Ethanol (2 minute per change).
13. Clear with 3 changes of xylene (5 minutes per change) and coverslip with Permount or other suitable organic mounting medium.

For Frozen Sections:

1. Fix frozen sections in 10% formalin (not Zinc) for 30 minutes.
2. Rinses in distilled water 3×3min.
3. Mordant in Bouin’s Solution, 60°C for 1 hour.
4. Wash in running tap water for 5 minutes to remove the picric acid.
5. Weigert’s working hematoxylin for 7 minutes. (Note: Discard after use.)
6. Blue in running tap water for 5 minutes, rinse in distilled water.
7. Biebrich Scarlet-Acid Fuchsin Solution for 5 minutes. (Note: Solution may be used twice only, then discarded.)
8. Rinse in distilled water.
9. Phosphotungstic/phosphomolybdic Acid Solution for 10 minutes, discard solution.
10. Transfer directly into Aniline Blue Solution for 5 minutes.
11. Rinse once for 2-3 seconds in distilled water.
12. 1% Acetic acid for 2-3 seconds , discard solution.
13. Dehydrate with 2 dip of 95% Ethanol and 2 changes of 100% Ethanol (2 minutes per change).
14. Clear with 3 changes of xylene (5 minutes per change) and coverslip with Permount or other suitable organic mounting medium.

Expected results

• Cytoplasm, keratin, muscle fibers, Erythrocytes ——— red
• Nuclei ———————————————————————— black
• Collagen and mucus ————————————————- blue

More Images

Masson Trichrome Staining, Rat Heart	Masson Trichrome Staining, Mouse Lung
	Masson Trichrome Staining, Mouse Esophagus

Masson’s Trichrome Stain Kit Comparison Among Different Vendors

Vendor / Catalog#	VitroVivo Biotech / VB-3016	Abcam / ab150686	Millipore-Sigma / HT15
Components	1. Bouin’s Solution (1 x 250 mL) 2. Weigert’s Hematoxylin Solution A (1 x 125 mL) 3. Weigert’s Hematoxylin Solution B (1 x 125 mL) 4. Biebrich Scarlet-Acid Fuchsin Solution (1 x 250 mL) 5. Phosphomolybdic-Phosphotungstic Acid Solution (1 x 250 mL) 6. Aniline Blue Solution (1 x 250 mL) 7. 1% Acetic Acid Solution (1 x 250 mL)	1. 1% Acetic Acid Solution (1 x 125 mL) 2. Aniline Blue Solution (1 x 125 mL) 3. Biebrich Scarlet/Acid Fuchsin Solution (1 x 125 mL) 4. Bouin’s Fluid (1 x 125 mL) 5. Phosphomolybdic/Phosphotungstic Acid Solution (1 x 125 mL) 6. Weigert’s Iron Hematoxylin A (1 x 125 mL) 7. Weigert’s Iron Hematoxylin B (1 x 125 mL)	1. Biebrich Scarlet-Acid Fuchsin Solution (Cat. No. HT151-250ML) 2. Phosphotungstic Acid Solution (Cat. No. HT152-250ML) 3. Phosphomolybdic Acid Solution (Cat. No. HT153-250ML) 4. Aniline Blue Solution (Cat. No. HT154-250ML)
Website	https://vitrovivo.com/product/massons-trichrome-stain-kit/	https://www.abcam.com/en-us/products/assay-kits/trichrome-stain-kit-connective-tissue-stain-ab150686	https://www.sigmaaldrich.com/US/en/product/sigma/ht15#product-documentation
List Price (USD)	$369 / kit	$505 / kit	$429 / kit
Tests per Kit (1 mL/slide)	250 slides	125 slides	250 slides
Cost per Slide (1 mL/slide)	$1.48 / slide	$4.04 / slide	$1.72 / slide, plus cost of other reagents
Quality/Staining Images

Publications that utilized the VitroView™ Masson’s Trichrome Stain Kit

1. Ko KW, et al. (2021). Integrated bioactive scaffold with polydeoxyribonucleotide and stem-cell-derived extracellular vesicles for kidney regeneration. ACS Biomaterials Science & Engineering, 15, 4, 7575–7585.
2. Park SY, et al. (2024). Multifunctional vitamin D-incorporated PLGA scaffold with BMP/VEGF-overexpressed tonsil-derived MSC via CRISPR/Cas9 for bone tissue regeneration. Materials Today Bio, 28: 101254.
3. Lee JK, et al. (2024). Osteoporotic bone regeneration via plenished biomimetic PLGA scaffold with sequential release system. Small, 20:2310734
4. Barkovskaya A, et al. (2025). Inhibition of PIKfyve kinase induces senescent cell death by suppressing lysosomal exocytosis and leads to improved outcomes in a mouse model of idiopathic pulmonary fibrosis. bioRxiv. https://doi.org/10.1101/2025.03.19.644224
5. Nam SH, et al. (2024). Glycinamide facilitates nanocomplex formation and functions synergistically with bone morphogenetic protein 2 to promote osteoblast differentiation in vitro and in vivo. Tissue Engineering and Regenerative Medicine, 21: 1093–1107
6. Scalco A, et al. (2024). Hypertension-induced heart failure disrupts cardiac sympathetic innervation. American Journal of Physiology, 327: H1544-H1558
7. Jung JW, et al. (2023). Advanced α-CSH/β-TCP-based injectable paste with magnesium hydroxide and vitamin D-incorporated PLGA microspheres for bone repair. Materials Today, 20: 100447
8. Lee JK, et al. (2023). Nitric oxide‐releasing bioinspired scaffold for exquisite regeneration of osteoporotic bone via regulation of homeostasis. Advanced Healthcare Materials, 10: 2205336
9. Park H, et al. (2024). Evaluation of liver function using Cordyceps militaris extract powder in Sprague-Dawley rats with acute hepatic injury induced by dimethylnitrosamine. Korean Journal of Agricultural Science, 51:147-158
10. Maselli DB, et al. (2024). Endoscopic gastric fundal mucosal ablation induces weight loss in adults with obesity. Gastroenterology, 167: p1457-1459.e3
11. Heo Y, Shin SW, Kim DS, Lee S, Park SY, et al. (2022). Bioactive PCL microspheres with enhanced biocompatibility and collagen production for functional hyaluronic acid dermal fillers. Biomaterials Advances, 10: 947-959
12. Bui HTD,et al. . (2023). Korean amberjack skin-inspired hyaluronic acid bioink for reconstruction of human skin. ACS Omega, 8, 25, 22752–22761.
13. Kim DS, et al. (2022). Controlled vitamin D delivery with injectable hyaluronic acid-based hydrogel for restoration of tendinopathy. Journal of Tissue Engineering, 13:  doi:1177/20417314221122089
14. Kim DS, et al. (2021). Advanced PLGA hybrid scaffold with a bioactive PDRN/BMP2 nanocomplex for angiogenesis and bone regeneration using human fetal MSCs. Science Advances, 7: eabj1083.
15. Baek SW, et al. (2023). Continuous NO dual-generation by ZnO nanoparticle conjugated with α-lipoic acid for functional biodegradable vascular stent. Chemical Engineering Journal. 470: 144174
16. Lee S, et al. (2024). Injectable Microparticle-containing hydrogel with controlled release of bioactive molecules for facial rejuvenation. Materials Today Bio, 24:100890
17. Meca-Laguna G, et al. (2025). γδ T Cells Target and Ablate Senescent Cells in Aging and Alleviate Pulmonary Fibrosis. bioRxiv preprint doi: https://doi.org/10.1101/2025.05.05.652251

Why Choose the VitroView™ Masson’s Trichrome Stain Kit for Your Research?

1. Cost-effective
2. Easy to use
3. High quality with strict quality control and assurance
4. Validated and trusted by numerous peer-reviewed publications

 

Note: This Trichrome Stain Kit is intended for research purposes only. This product is not intended to be used for therapeutic or diagnostic purposes in humans or animals.

Precautions: Handle with care. Avoid contact with eyes, skin and clothing. Do not ingest. Wear gloves.

User Manual and Material Safety Data Sheet (MSDS)  (PDF)

VB-3016 User manual

VB-3016 MSDS