Description
Andy Fluor™ 594 Goat Anti-Rabbit IgG (H+L) Antibody is prepared by labeling high quality goat anti-rabbit IgG (H+L) with Andy Fluor™ 594. Andy Fluor™ 594 is superior in fluorescent brightness and photostability. Andy Fluor™ 594 can be used as a replacement for Alexa Fluor® 594 or Texas Red® dye.
Features
| Form Information | Specifications | |||
| Physical State | Lyophilized powder | Host/Isotype | Goat | |
| Shipping Condition | Ambient | Class & Form | Polyclonal/ Whole Antibody | |
| Purification | Immunoaffinity chromatography | Type | Secondary Antibody | |
| Buffer | PBS, pH 7.4, with 0.1% BSA | Tested Species Reactivity | Rabbit (Rb) | |
| Preservative | 0.02% sodium azide | Target Class | IgG (H+L) | |
| Storage Conditions | 2-8ºC; store in dark | Label | Andy Fluor™ 594 | |
| Package size | 500 µg /vial | Excitation/Emission |
590/615 nm | |
Reconstitution and Storage
Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
Applications
- Western Blot (WB) 1:5,000 – 1:20,000
- Immunocytochemistry (ICC) 1:100 – 1:2,000
- Immunohistochemistry (IHC) 1:100 – 1:2,000
- Flow Cytometry (FACS) 1:100 – 1:1,000
