Andy Fluor™ 488 Streptavidin


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SKU: VB-L156B Category:


Andy Fluor™ 488 streptavidin is prepared by covalently labeling streptavidin with Andy Fluor™ 488 dye. Streptavidin has high binding affinity with biotin, so streptavidin conjugate is commonly used together with biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules. Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.


Form Information   Specifications
Physical State Lyophilized power   Host/Isotype  
Shipping Condition Ambient   Class & Form  
Purification Immunoaffinity chromatography   Type Streptavidin
Buffer PBS, pH 7.4, with 0.1% BSA   Tested Reactivity Biotin
Preservative 0.02% sodium azide   Target Class  
Storage Conditions 2-8ºC; store in dark   Label Andy Fluor 488
Package size 500 µg /vial   Excitation/Emission 
495/520 nm

Reconstitution and Storage

Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.


  • Western Blot (WB)  1:5,000 – 1:20,000
  • Immunocytochemistry (ICC) 1:100 – 1:2,000
  • Immunohistochemistry (IHC) 1:100 – 1:2,000
  • Flow Cytometry (FACS) 1:100 – 1:1,000

User Manual (PDF)

VB-L156B user manual