Andy Fluor™ 488 Streptavidin

$80.00

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SKU: VB-L156B Category:

Description

Andy Fluor™ 488 streptavidin is prepared by covalently labeling streptavidin with Andy Fluor™ 488 dye. Streptavidin has high binding affinity with biotin, so streptavidin conjugate is commonly used together with biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules. Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.

Features

Form Information Specifications
Physical StateLyophilized power Host/Isotype 
Shipping ConditionAmbient Class & Form 
PurificationImmunoaffinity chromatography TypeStreptavidin
BufferPBS, pH 7.4, with 0.1% BSA Tested ReactivityBiotin
Preservative0.02% sodium azide Target Class 
Storage Conditions2-8ºC; store in dark LabelAndy Fluor 488
Package size500 µg /vial Excitation/Emission 
495/520 nm

Reconstitution and Storage

Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.

Applications

  • Western Blot (WB)  1:5,000 – 1:20,000
  • Immunocytochemistry (ICC) 1:100 – 1:2,000
  • Immunohistochemistry (IHC) 1:100 – 1:2,000
  • Flow Cytometry (FACS) 1:100 – 1:1,000

User Manual (PDF)

VB-L156B user manual