Description
Andy Fluor™ 488 streptavidin is prepared by covalently labeling streptavidin with Andy Fluor™ 488 dye. Streptavidin has high binding affinity with biotin, so streptavidin conjugate is commonly used together with biotin conjugate for specific detection of a variety of proteins, protein motifs, nucleic acids, and other molecules. Strategies similar to this are used in many detection protocols including western blots, flow cytometry, imaging and microscopy, and microplate assays.
Features
| Form Information | Specifications | |||
| Physical State | Lyophilized power | Host/Isotype | ||
| Shipping Condition | Ambient | Class & Form | ||
| Purification | Immunoaffinity chromatography | Type | Streptavidin | |
| Buffer | PBS, pH 7.4, with 0.1% BSA | Tested Reactivity | Biotin | |
| Preservative | 0.02% sodium azide | Target Class | ||
| Storage Conditions | 2-8ºC; store in dark | Label | Andy Fluor™ 488 | |
| Package size | 500 µg /vial | Excitation/Emission |
495/520 nm | |
Reconstitution and Storage
Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
Applications
- Western Blot (WB) 1:5,000 – 1:20,000
- Immunocytochemistry (ICC) 1:100 – 1:2,000
- Immunohistochemistry (IHC) 1:100 – 1:2,000
- Flow Cytometry (FACS) 1:100 – 1:1,000
User Manual (PDF)
