Andy Fluor™ 350 Goat Anti-Rabbit IgG (H+L) Antibody

$80.00

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Description

Andy Fluor™ 350 Goat Anti-Rabbit IgG (H+L) Antibody is prepared by labeling high quality goat anti-mouse IgG (H+L) with Andy Fluor™ 350. Andy Fluor™ 350 is a blue fluorescent dye spectrally similar to the traditional blue fluorescent dye, AMCA. However, compared to AMCA, Andy Fluor™ 350 is more water-soluble, more photostable and more fluorescent on proteins. Andy Fluor™ 350 can be used as a replacement for Alexa Fluor® 350.

Features

Form Information   Specifications
Physical State Lyophilized powder   Host/Isotype  Goat
Shipping Condition Ambient   Class & Form Polyclonal/ Whole Antibody
Purification Immunoaffinity chromatography   Type Secondary Antibody
Buffer PBS, pH 7.4, with 0.1% BSA   Tested Species Reactivity Rabbit (Rb)
Preservative 0.02% sodium azide   Target Class IgG (H+L)
Storage Conditions 2-8ºC; store in dark   Label Andy Fluor™ 350
Package size 500 µg /vial   Excitation/Emission 
 345/440 nm

Reconstitution and Storage

Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.

Applications

  • Western Blot (WB)  1:5,000 – 1:20,000
  • Immunocytochemistry (ICC) 1:100 – 1:2,000
  • Immunohistochemistry (IHC) 1:100 – 1:2,000
  • Flow Cytometry (FACS) 1:100 – 1:1,000

User Manual (PDF)

VB-L101B user manual