Andy Fluor™ 350 Goat Anti-Rabbit IgG (H+L) Antibody is prepared by labeling high quality goat anti-mouse IgG (H+L) with Andy Fluor™ 350. Andy Fluor™ 350 is a blue fluorescent dye spectrally similar to the traditional blue fluorescent dye, AMCA. However, compared to AMCA, Andy Fluor™ 350 is more water-soluble, more photostable and more fluorescent on proteins. Andy Fluor™ 350 can be used as a replacement for Alexa Fluor® 350.
|Physical State||Lyophilized powder||Host/Isotype||Goat|
|Shipping Condition||Ambient||Class & Form||Polyclonal/ Whole Antibody|
|Purification||Immunoaffinity chromatography||Type||Secondary Antibody|
|Buffer||PBS, pH 7.4, with 0.1% BSA||Tested Species Reactivity||Rabbit (Rb)|
|Preservative||0.02% sodium azide||Target Class||IgG (H+L)|
|Storage Conditions||2-8ºC; store in dark||Label||Andy Fluor™ 350|
|Package size||500 µg /vial||Excitation/Emission
Reconstitution and Storage
Store lyophilized power at 2-8ºC. When ready to use, rehydrate with dH2O (250 µL for 500 µg antibody) to make 2 mg/mL solution and centrifuge if not clear. Product is stable for about 6 months at 2-8ºC as an undiluted liquid. Prepare working dilution fresh each day. For extended storage after rehydration, add an equal volume of glycerol for a final concentration of 50%, and store at -20 ºC as a liquid.
- Western Blot (WB) 1:5,000 – 1:20,000
- Immunocytochemistry (ICC) 1:100 – 1:2,000
- Immunohistochemistry (IHC) 1:100 – 1:2,000
- Flow Cytometry (FACS) 1:100 – 1:1,000